Crystal Structure of the N-terminal Domain of Anaphase-promoting Complex Subunit
نویسندگان
چکیده
Dohyun Han, Kyunggon Kim, Yeonjung Kim, Yup Kang, Ji Yoon Lee, and Youngsoo Kim From the Department of Biomedical Sciences and Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, the Institute for Medical Sciences, Ajou University School of Medicine, Wonchon-dong San 5, Suwon, Kyunggi-do 442-749, and the National Instrumentation Center for Environmental Management, Seoul National University, Seoul 151-921, Korea
منابع مشابه
Atomic-Resolution Structures of the APC/C Subunits Apc4 and the Apc5 N-Terminal Domain
Many essential biological processes are mediated by complex molecular machines comprising multiple subunits. Knowledge on the architecture of individual subunits and their positions within the overall multimeric complex is key to understanding the molecular mechanisms of macromolecular assemblies. The anaphase-promoting complex/cyclosome (APC/C) is a large multisubunit complex that regulates ce...
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In mitosis, the spindle checkpoint detects a single unattached kinetochore, inhibits the anaphase-promoting complex or cyclosome (APC/C), and prevents premature sister chromatid separation. The checkpoint kinase Bub1 contributes to checkpoint sensitivity through phosphorylating the APC/C activator, Cdc20, and inhibiting APC/C catalytically. We report here the crystal structure of the kinase dom...
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The anaphase-promoting complex/cyclosome (APC/C) is a large multimeric cullin-RING E3 ubiquitin ligase that orchestrates cell-cycle progression by targeting cell-cycle regulatory proteins for destruction via the ubiquitin proteasome system. The APC/C assembly comprises two scaffolding subcomplexes: the platform and the TPR lobe that together coordinate the juxtaposition of the catalytic and sub...
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The execution of meiotic divisions in Saccharomyces cerevisiae is regulated by anaphase-promoting complex/cyclosome (APC/C)-mediated protein degradation. During meiosis, the APC/C is activated by association with Cdc20p or the meiosis-specific activator Ama1p. We present evidence that, as cells exit from meiosis II, APC/C(Ama1) mediates Cdc20p destruction. APC/C(Ama1) recognizes two degrons on ...
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